PLDN Polyklonaler Antikörper

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PLDN Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10891-2-PBS

Synonyme

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  WB analysis using 10891-2-AP (same clone as 10891-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10891-2-AP (PLDN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10891-2-AP (same clone as 10891-2-PBS)

  WB result of PLDN antibody (10891-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLDN transfected A431 cells. This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 10891-2-AP (same clone as 10891-2-PBS)

  IP result of anti-PLDN (IP:10891-2-AP, 3ug; Detection:10891-2-AP 1:500) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10891-2-AP (same clone as 10891-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10891-2-AP (PLDN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10891-2-AP (same clone as 10891-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10891-2-AP (PLDN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10891-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10891-2-PBS bindet in WB, IHC, IP, Indirect ELISA PLDN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLDN fusion protein Ag1337
• Vollständiger Name: pallidin homolog (mouse)
• Berechnetes Molekulargewicht: 23~24 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC004819
• Gene symbol: PLDN
• Gene ID (NCBI): 26258
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pallidin, also named as PLDN and PA, is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. It may play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process. Pallidin interacts with syntaxin-12 which is a t-SNARE protein that mediates vesicle docking and fusion. Pallidin is a 25 kDa subunit of the octamer BLOC-1.