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PLEC Polyklonaler Antikörper

PLEC Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29170-1-PBS

Synonyme

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

Filter:
  • Western Blot (WB) analysis of various lysates using PLEC Polyclonal antibody (29170-1-AP)

    WB analysis using 29170-1-AP (same clone as 29170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29170-1-AP (PLEC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PLEC Polyclonal antibody (29170-1-AP)

    IHC staining of mouse heart using 29170-1-AP (same clone as 29170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29170-1-AP (PLEC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PLEC Polyclonal antibody (29170-1-AP)

    IF Staining of A549 using 29170-1-AP (same clone as 29170-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEC antibody (29170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29170-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PLEC und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PLEC fusion protein Ag29418
Vollständiger Name plectin 1, intermediate filament binding protein 500kDa
Berechnetes Molekulargewicht 532 kDa
Beobachtetes Molekulargewicht 510 kDa
GenBank-ZugangsnummerNM_201380
Gene symbol Plectin
Gene ID (NCBI) 5339
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)