PLEK Polyklonaler Antikörper

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• KD/KO Validated

PLEK Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12506-1-AP

Synonyme

P47, Platelet 47 kDa protein, pleckstrin, PLEK

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Galerie der Validierungsdaten

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  WB analysis of U-937 using 12506-1-AP

  WB result of PLEK antibody (12506-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLEK transfected U-937 cells.

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  WB analysis of U-937 using 12506-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12506-1-AP (PLEK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 12506-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 12506-1-AP (PLEK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 12506-1-AP

  IP Result of anti-PLEK (IP:12506-1-AP, 3ug; Detection:12506-1-AP 1:1000) with U-937 cells lysate 3000ug.

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  IHC staining of human lung cancer using 12506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12506-1-AP (PLEK antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

12506-1-AP bindet in WB, IP, IHC PLEK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLEK fusion protein Ag3187
• Vollständiger Name: pleckstrin
• Berechnetes Molekulargewicht: 350 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40-47 kDa
• GenBank-Zugangsnummer: BC018549
• Gene symbol: PLEK
• Gene ID (NCBI): 5341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

Publikationen

Species	Application	Title
human	WB	Mol Med; DMAG, a novel countermeasure for the treatment of thrombocytopenia.; Authors - Jing Lin
human,mouse	WB	Front Neurosci; MiR-409-5p as a Regulator of Neurite Growth Is Down Regulated in APP/PS1 Murine Model of Alzheimer's Disease.; Authors - Jing Guo ; KD Validated
human	WB	mSphere; Ehrlichia chaffeensis TRP75 Interacts with Host Cell Targets Involved in Homeostasis, Cytoskeleton Organization, and Apoptosis Regulation To Promote Infection.; Authors - Tian Luo