PLEKHA1 Polyklonaler Antikörper

PLEKHA1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10238-1-AP

Synonyme

PLEKHA1, TAPP 1, TAPP1

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Galerie der Validierungsdaten

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  WB analysis of human placenta using 10238-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10238-1-AP (PLEKHA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10238-1-PBS

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10238-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human colon using 10238-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10238-1-AP (PLEKHA1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 10238-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10238-1-AP (PLEKHA1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 10238-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10238-1-AP (PLEKHA1 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of HeLa using 10238-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10238-1-AP (PLEKHA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10238-1-AP bindet in WB, IHC, IF, ELISA PLEKHA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLEKHA1 fusion protein Ag0318
• Vollständiger Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC001136
• Gene symbol: PLEKHA1
• Gene ID (NCBI): 59338
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein- tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1."

Publikationen

Species	Application	Title
mouse	WB	J Exp Med; Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.; Authors - Michelle E LeBlanc
mouse		Exp Eye Res; Function-first ligandomics for ocular vascular research and drug target discovery.; Authors - Xin Rong