PLEKHA2 Polyklonaler Antikörper

PLEKHA2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32169-1-AP

Synonyme

TAPP-2, TAPP2, Tandem PH domain-containing protein 2, Pleckstrin homology domain-containing family A member 2, Pleckstrin Homology Domain Containing A2

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  WB analysis using 32169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32169-1-AP (PLEKHA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 32169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HeLa using 32169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32169-1-AP bindet in WB, IF/ICC, ELISA PLEKHA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLEKHA2 fusion protein Ag36596
• Vollständiger Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 2
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC166639
• Gene symbol: PLEKHA2
• Gene ID (NCBI): 59339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLEKHA2 (Pleckstrin homology domain containing A2), also known as TAPP2, is a member of the PLEKHA family of proteins.PLEKHA2 is a junctional protein with a Pleckstrin homology domain that is involved in signaling after lymphocyte activation and plays an important role in cytoskeletal reorganization driven by phosphatidylinositol 3-kinase.