Plasminogen Polyklonaler Antikörper

Plasminogen Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17462-1-AP

Synonyme

PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin

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  WB analysis of human blood using 17462-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17462-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17462-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 17462-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17462-1-AP (Plasminogen antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17462-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17462-1-AP (Plasminogen antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

17462-1-AP bindet in WB, IHC, IF/ICC, ELISA Plasminogen und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Plasminogen fusion protein Ag11217
• Vollständiger Name: plasminogen
• Berechnetes Molekulargewicht: 810 aa, 91 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC060513
• Gene symbol: Plasminogen
• Gene ID (NCBI): 5340
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

Publikationen

Species	Application	Title
		Front Microbiol; Binding of Plasminogen to Streptococcus suis Protein Endopeptidase O Facilitates Evasion of Innate Immunity in Streptococcus suis.; Authors - Yang Zhou
mouse	WB	Blood; A mechanism for hereditary angioedema with normal C1 inhibitor: an inhibitory regulatory role for the factor XII heavy chain; Authors - Ivan Ivanov
mouse	WB	Nat Commun; Fibrinolytic-deficiencies predispose hosts to septicemia from a catheter-associated UTI; Authors - Jonathan J Molina