Perilipin 1 Polyklonaler Antikörper

Perilipin 1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 27716-1-AP

Synonyme

PLIN, PERI, PLIN1

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  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (Perilipin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (PLIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 27716-1-AP

  IP result of anti-Perilipin 1 (IP:27716-1-AP, 4ug; Detection:27716-1-AP 1:2000) with NIH/3T3 cells lysate 1505 ug.

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  IHC staining of mouse brown adipose using 27716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 27716-1-AP (Perilipin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 27716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 27716-1-AP (Perilipin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 3T3-L1-Zellen, MCF-7-Zellen, Mauslebergewebe, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: braunes Mausfettgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: braunes Mausfettgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

27716-1-AP bindet in WB, IHC, IF-P, IP, ELISA Perilipin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Perilipin 1 fusion protein Ag26426
• Vollständiger Name: perilipin
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 62-67 kDa
• GenBank-Zugangsnummer: NM_001145311
• Gene symbol: Perilipin 1
• Gene ID (NCBI): 5346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).

Publikationen

Species	Application	Title
mouse	WB	Hum Gene Ther; Kallistatin Improves High-Fat-Induced Insulin Resistance via Epididymal Adipose Tissue-Derived Exosomes; Authors - Zi-Wei Yang
mouse,human	WB	Metabolism; Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease; Authors - Jiang Du
mouse	IHC	Cells; Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells; Authors - Taeko Ichise
goat	WB	Biol Reprod; NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells†; Authors - Hao Yu
mouse	IF	J Plast Reconstr Aesthet Surg; Poloxamer 188 washing of lipoaspirate improves fat graft survival: A comparative study in nude mice; Authors - Meiling Liu
sheep	WB	Front Vet Sci; Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes; Authors - Wei Zhang