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PLK1 Polyklonaler Antikörper

PLK1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12098-1-AP

Synonyme

PLK, PLK 1, PLK1, Polo like kinase 1, STPK13

Galerie der Validierungsdaten

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLK1 Polyclonal antibody (12098-1-AP)

    IHC staining of human colon cancer using 12098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12098-1-AP (PLK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLK1 Polyclonal antibody (12098-1-AP)

    IHC staining of human colon cancer using 12098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12098-1-AP (PLK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, U2OS-Zellen
Erfolgreiche IPMaushodengewebe
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12098-1-AP bindet in WB, IP, IHC,ELISA PLK1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PLK1 fusion protein Ag2742
Vollständiger Name polo-like kinase 1 (Drosophila)
Berechnetes Molekulargewicht 603 aa, 68 kDa
GenBank-ZugangsnummerBC014846
Gene symbol PLK1
Gene ID (NCBI) 5347
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).