PLK2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PLK2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15956-1-AP

Synonyme

PLK 2, PLK2, Polo like kinase 2, Serum inducible kinase, SNK

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Galerie der Validierungsdaten

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  WB analysis using 15956-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15956-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15956-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 15956-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15956-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15956-1-AP

  IP Result of anti-PLK2 (IP:15956-1-AP, 4ug; Detection:15956-1-AP 1:300) with HeLa cells lysate 2480ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

15956-1-AP bindet in WB, IP,ELISA PLK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLK2 fusion protein Ag8710
• Vollständiger Name: polo-like kinase 2 (Drosophila)
• Berechnetes Molekulargewicht: 685 aa, 78 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC013879
• Gene symbol: PLK2
• Gene ID (NCBI): 10769
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).

Publikationen

Species	Application	Title
human	WB	Oncotarget; MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting polo-like kinase2 in cervical cancer.; Authors - Fei Liu
rat	WB	J Biochem Mol Toxicol; PLK2 protects retinal ganglion cells from oxidative stress by potentiating Nrf2 signaling via GSK-3β.; Authors - Yazhi Fan ; KD Validated
	IP	Cell Biol Int; Tazarotene-induced gene 1 interacts with Polo-like kinase 2 and inhibits cell proliferation in HCT116 colorectal cancer cells.; Authors - Chun-Hua Wang