PLK3 Polyklonaler Antikörper

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• KD/KO Validated

PLK3 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 10977-1-AP

Synonyme

CNK, Cytokine-inducible serine/threonine-protein kinase, EC:2.7.11.21, FGF-inducible kinase, FNK

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  IHC staining of mouse testis using 10977-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10977-1-AP (PLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10977-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10977-1-AP (PLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10977-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10977-1-AP (PLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10977-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10977-1-AP (PLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

10977-1-AP bindet in IHC, IF, ELISA PLK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLK3 fusion protein Ag1431
• Vollständiger Name: polo-like kinase 3 (Drosophila)
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC013899
• Gene symbol: PLK3
• Gene ID (NCBI): 1263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53(PMID:12548019).

Publikationen

Species	Application	Title
human	IHC	Liver Int; Polo-like kinase 3 is associated with improved overall survival in cholangiocarcinoma.; Authors - Benjamin Juntermanns
human	IHC	Clin Experiment Ophthalmol; Prognostic significance of polo-like kinases in retinoblastoma: correlation with patient outcome, clinical and histopathological parameters.; Authors - Lata Singh
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
		Mol Cancer; LINC00115 promotes chemoresistant breast cancer stem-like cell stemness and metastasis through SETDB1/PLK3/HIF1α signaling; Authors - Fei Luo
mouse,human	IHC	Oncol Res; Comprehensive analysis reveals PLK3 as a promising immune target and prognostic indicator in glioma; Authors - Tianyun Zhu