PLK4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PLK4 Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28750-1-PBS

Synonyme

---

• 

  IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 28750-1-AP (same clone as 28750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 28750-1-AP (same clone as 28750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 28750-1-AP (same clone as 28750-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using PLK4 antibody (28750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

28750-1-PBS bindet in IHC, IF/ICC, Indirect ELISA PLK4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLK4 fusion protein Ag30482
• Vollständiger Name: polo-like kinase 4 (Drosophila)
• Berechnetes Molekulargewicht: 970 aa, 109 kDa
• GenBank-Zugangsnummer: BC036023
• Gene symbol: PLK4
• Gene ID (NCBI): 10733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.