PLOD1 Polyklonaler Antikörper

PLOD1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 29480-1-AP

Synonyme

FLJ42041, LH, LH1, LLH, Lysyl hydroxylase 1, PLOD, PLOD1

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  WB analysis using 29480-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 29480-1-AP (PLOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29480-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29480-1-AP (PLOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29480-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 29480-1-AP (PLOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 29480-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 29480-1-AP (PLOD1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 29480-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 29480-1-AP (PLOD1 antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of K-562 using 29480-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using PLOD1 antibody (29480-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, K-562-Zellen, U-251-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

29480-1-AP bindet in WB, IHC, IF/ICC, ELISA PLOD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLOD1 fusion protein Ag31626
• Vollständiger Name: procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
• Berechnetes Molekulargewicht: 727 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84-88 kDa
• GenBank-Zugangsnummer: BC016657
• Gene symbol: PLOD1
• Gene ID (NCBI): 5351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLOD1, as known as procollagen‐lysine, 2‐oxoglutarate 5‐dioxygenase 1, is a member of the PLOD family(Uniprot, GeneID:5351). PLOD1 participates in catalyzing hydroxylysine residue, which is critical for the formation of covalent cross-link (PMID:30003082). PLOD1 plays an important role in the development and progression of tumors. Aberrantly expressed PLOD1 promotes cancer aggressiveness in glioma and bladder cancer, providing a potential prognostic biomarker and therapeutic target (PMID:31199049, PMID:34040895). PLOD1 has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	IHC	Aging (Albany NY); Systematic characterization of the expression, prognosis and immune characteristics of PLOD family genes in breast cancer; Authors - Dan-Dan Wang
human	WB,IF	Phytomedicine; Gluconic acid alleviates hypertrophic scar formation through binding PLOD1, reducing p-AKT signaling and activating autophagy; Authors - Jingyun Li