PLOD2 Monoklonaler Antikörper

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• KD/KO Validated

PLOD2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgA

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1H9E1

Kat-Nr. : 66342-1-Ig

Synonyme

LH2, Lysyl hydroxylase 2, PLOD2, TLH

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66342-1-Ig

  WB result of PLOD2 antibody (66342-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2 transfected HEK-293 cells.

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  WB analysis using 66342-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 66342-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66342-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, 4T1-Zellen, A431-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

66342-1-Ig bindet in WB, IHC, ELISA PLOD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgA
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLOD2 fusion protein Ag5779
• Vollständiger Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
• Berechnetes Molekulargewicht: 758 aa, 85 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC037169
• Gene symbol: PLOD2
• Gene ID (NCBI): 5352
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy.(PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.

Publikationen

Species	Application	Title
human	WB,IHC	Front Cell Dev Biol; Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.; Authors - Yajuan Zhao
human	WB	J Invest Dermatol; Loss of RhoE function in dermatofibroma promotes disorganized dermal fibroblast extracellular matrix and increased integrin activation; Authors - Sofia Endzhievskaya ; KD Validated
human	WB,IHC	Cell Death Dis; Hypoxia-induced PLOD2 promotes clear cell renal cell carcinoma progression via modulating EGFR-dependent AKT pathway activation; Authors - Tao Liu ; KD Validated