PLRG1 Polyklonaler Antikörper

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• KD/KO Validated

PLRG1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11914-1-PBS

Synonyme

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  WB analysis of K-562 using 11914-1-AP (same clone as 11914-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

  WB result of PLRG1 antibody (11914-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLRG1 transfected HEK-293 cells. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 11914-1-AP (same clone as 11914-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

  IP result of anti-PLRG1 (IP:11914-1-AP, 4ug; Detection:11914-1-AP 1:1000) with HEK-293 cells lysate 1400ug. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 11914-1-AP (same clone as 11914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 11914-1-AP (same clone as 11914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11914-1-PBS bindet in WB, IHC, IP, Indirect ELISA PLRG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLRG1 fusion protein Ag2527
• Vollständiger Name: pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
• Berechnetes Molekulargewicht: 514 aa, 57 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC020786
• Gene symbol: PLRG1
• Gene ID (NCBI): 5356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLRG1, identifed as a component of the CDC5L complex, is one spliceosomal protein highly conserved across species. It has been shown to be essential for pre-mRNA splicing in yeast and humans.