PLS3 Rekombinanter Antikörper

PLS3 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250908B6

Kat-Nr. : 86323-1-RR

Synonyme

Plastin 3, Plastin-3, T plastin, T-fimbrin, T-plastin

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  WB analysis using 86323-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86323-1-RR (PLS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86323-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86323-1-RR (PLS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 86323-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86323-1-RR (PLS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 86323-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86323-1-RR (PLS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, COLO 320-Zellen, HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86323-1-RR bindet in WB, IHC, ELISA PLS3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: plastin 3 (T isoform)
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: NM_005032
• Gene symbol: PLS3
• Gene ID (NCBI): 5358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLS3, also named T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. L-plastin plays an important role in leukocyte function, and T-plastin is possibly involved in DNA repair. Both T- and L-plastin are implicated in several diseases, and L-plastin is considered to be a valuable marker for cancer (PMID: 15960882).