PLSCR1 Rekombinanter Antikörper

PLSCR1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242372B2

Kat-Nr. : 84888-4-RR

Synonyme

phospholipid scramblase 1, MMTRA1B, Mg(2+)-dependent nuclease, EC:3.1.-.-, Ca(2+)-dependent phospholipid scramblase 1

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  WB analysis using 84888-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84888-4-RR (PLSCR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84888-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84888-4-RR against Human PLSCR1 were performed. The affinity constant is 0.377 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, HEK-293-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84888-4-RR bindet in WB, ELISA PLSCR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PLSCR1 fusion protein Ag2172
• Vollständiger Name: phospholipid scramblase 1
• Berechnetes Molekulargewicht: 318 aa, 35 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC021100
• Gene symbol: PLSCR1
• Gene ID (NCBI): 5359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).