PLUNC Polyklonaler Antikörper

PLUNC Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10413-1-AP

Synonyme

BPIFA1, LPLUNC3, Lung specific protein X, LUNX, NASG, PLUNC, Protein Plunc, SPLUNC1, SPURT, Von Ebner protein Hl

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 10413-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10413-1-AP (PLUNC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10413-1-AP

  IP Result of anti-PLUNC (IP:10413-1-AP, 4ug; Detection:10413-1-AP 1:300) with mouse lung tissue lysate 3600ug.

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  IHC staining of mouse lung using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

10413-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA PLUNC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLUNC fusion protein Ag0629
• Vollständiger Name: palate, lung and nasal epithelium associated
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC012549
• Gene symbol: PLUNC
• Gene ID (NCBI): 51297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLUNC, also named as LUNX, NASG and SPURT, belongs to the BPI/LBP/Plunc superfamily and Plunc family. PLUNC may be involved in the airway inflammatory response after exposure to irritants. It is associated with tumor progression. PLUNC plays a role in innate immune responses of the upper airways.

Publikationen

Species	Application	Title
mouse	IF	Sci Rep; Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis; Authors - Chen-Qian Zhao
mouse,human	WB,IHC,IF,ChIP	Cell Death Dis; USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24; Authors - Huai Liu
human	WB	Med Oncol; SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells; Authors - Ling Tang