PLVAP Polyklonaler Antikörper

PLVAP Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 14452-1-AP

Synonyme

FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein, PV 1

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  WB analysis using 14452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14452-1-AP (PLVAP antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:9000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

14452-1-AP bindet in WB, IF, ELISA PLVAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLVAP fusion protein Ag5963
• Vollständiger Name: plasmalemma vesicle associated protein
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC056414
• Gene symbol: PLVAP
• Gene ID (NCBI): 83483
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plasmalemma vesicle-associated protein (PLVAP) is an endothelial-associated protein that is involved in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, PLVAP has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry into soluble ags into lymph node conduits .(PMID: 36884119, PMID: 37810232)

Publikationen

Species	Application	Title
mouse	WB	Neuron; The Wnt Inhibitor Apcdd1 Coordinates Vascular Remodeling and Barrier Maturation of Retinal Blood Vessels.; Authors - Jenna Mazzoni
pig	WB	J Virol; Porcine Epidemic Diarrhea Virus Infection Disrupts the Nasal Endothelial Barrier To Favor Viral Dissemination.; Authors - Jianda Li
human	WB	Front Pharmacol; Stimulatory G-Protein α Subunit Modulates Endothelial Cell Permeability Through Regulation of Plasmalemma Vesicle-Associated Protein.; Authors - Lifan He
mouse	WB	Biochem Biophys Res Commun; Retinal VEGFA maintains the ultrastructure and function of choriocapillaris by preserving the endothelial PLVAP.; Authors - Sang A Kim
		bioRxiv; Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling; Authors - Saptarshi Biswas
mouse	WB	Neuron; Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling; Authors - Saptarshi Biswas

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Michelle (Verified Customer) (12-01-2023)

This antibody works very well for PLVAP westernblot analysis. We detected strong und specific PLVAP bands, even with this relatively high dilution of 1:4500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4500
• Cell Tissue Type: cultured bEnd5 cells