PLVAP Monoklonaler Antikörper

PLVAP Monoklonal Antikörper für IF-P, IF-Fro, FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF-P, IF-Fro, FC

Konjugation

Unkonjugiert

CloneNo.

MECA-32

Kat-Nr. : 65214-1-Ig

Synonyme

MECA32, MECA-32, Panendothelial cell antigen, Plasmalemma vesicle protein 1, PV 1

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  IF Staining of mouse small intestine using 65214-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using PLVAP antibody (65214-1-Ig, Clone: MECA-32 ) at dilution of 1:250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 65214-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using PLVAP antibody (65214-1-Ig, Clone: MECA-32 ) at dilution of 1:250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of bEnd.3 using 65214-1-Ig

  1X10^6 bEnd.3 cells were surface stained with 0.5 ug Anti-Mouse Plvap (65214-1-Ig, Clone:MECA-32) (red), or 0.5 ug Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in FC: bEnd.3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:125-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:125-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65214-1-Ig bindet in IF-P, IF-Fro, FC PLVAP und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Mouse lymph node stromal cells
• Vollständiger Name: plasmalemma vesicle associated protein
• GenBank-Zugangsnummer: BC013517
• Gene symbol: Plvap
• Gene ID (NCBI): 84094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).