PLVAP Monoklonaler Antikörper

PLVAP Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

MECA-32

Kat-Nr. : CL750-65214

Synonyme

MECA32, Plvap, PV 1

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  FC experiment of bEnd.3 using CL750-65214

  1X10^6 bEnd.3 cells were surface stained with 0.5 ug CoraLite® Plus 750 Anti-Mouse PLVAP (CL750-65214, Clone:MECA-32) (red), or 0.5 ug CoraLite® Plus 750 Rat IgG2a Isotype Control (2A3) (CL750-65209, Clone: 2A3) (blue). Cells were not fixed.

• 

  FC experiment of bEnd.3 using CL750-65214

  1X10^6 bEnd.3 cells were surface stained with 0.5 ug CoraLite® Plus 750 Anti-Mouse PLVAP (CL750-65214, Clone:MECA-32), or 0.5 ug CoraLite® Plus 750 Rat IgG2a Isotype Control (2A3) (CL750-65209, Clone: 2A3). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: bEnd.3-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL750-65214 bindet in FC PLVAP und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Mouse lymph node stromal cells
• Vollständiger Name: plasmalemma vesicle associated protein
• GenBank-Zugangsnummer: BC013517
• Gene symbol: Plvap
• Gene ID (NCBI): 84094
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).