PLXNB2 Monoklonaler Antikörper

PLXNB2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G9B2

Kat-Nr. : 67265-1-PBS

Synonyme

dJ402G11.3, KIAA0315, MM1, Nbla00445, PLEXB2, plexin B2, PLXNB2

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  WB analysis using 67265-1-Ig (same clone as 67265-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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  WB analysis of ATDC-5 using 67265-1-Ig (same clone as 67265-1-PBS)

  ATDC-5 cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67265-1-Ig (same clone as 67265-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67265-1-Ig (same clone as 67265-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67265-1-Ig (same clone as 67265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67265-1-Ig (same clone as 67265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67265-1-PBS bindet in WB, IHC, Indirect ELISA PLXNB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PLXNB2 fusion protein Ag28706
• Vollständiger Name: plexin B2
• Berechnetes Molekulargewicht: 205 kDa
• Beobachtetes Molekulargewicht: 240 kDa
• GenBank-Zugangsnummer: NM_012401
• Gene symbol: PLXNB2
• Gene ID (NCBI): 23654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 20-466aa of human plexin B2 can recognize precursor and alpha subunit of plexin B2.