PMEPA1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PMEPA1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 16521-1-AP

Synonyme

Transmembrane prostate androgen-induced protein, TMEPAI, STAG1, Solid tumor-associated 1 protein, Protein TMEPAI

---

• 

  WB analysis using 16521-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16521-1-AP (PMEPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 16521-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16521-1-AP (PMEPA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16521-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16521-1-AP (PMEPA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

16521-1-AP bindet in WB, IHC, IF, ELISA PMEPA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PMEPA1 fusion protein Ag9744
• Vollständiger Name: prostate transmembrane protein, androgen induced 1
• Berechnetes Molekulargewicht: 287 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-36 kDa
• GenBank-Zugangsnummer: BC015918
• Gene symbol: PMEPA1
• Gene ID (NCBI): 56937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMEPA1, also known as TMEPAI, was first discovered as androgen inducible gene in hormone responsive LNCaP cells. PMEPA1 is highly expressed in various solid tumors and is known to play important roles in the transforming growth factor-β(TGF-β) signaling pathway (PMID: 27697531).

Publikationen

Species	Application	Title
human	WB	EMBO Mol Med; An autocrine ActivinB mechanism drives TGFβ/Activin signaling in Group 3 medulloblastoma.; Authors - Morgane Morabito
human,mouse	WB,IHC,IF	Int J Biol Sci; METTL16 suppressed the proliferation and cisplatin-chemoresistance of bladder cancer by degrading PMEPA1 mRNA in a m6A manner through autophagy pathway; Authors - Hao Yu
human	WB,IHC	Front Immunol; PMEPA1 Is a Prognostic Biomarker That Correlates With Cell Malignancy and the Tumor Microenvironment in Bladder Cancer; Authors - Dongxu Qiu ; KD Validated
mouse	IF	FASEB J; PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking.; Authors - Hirohito Hirata ; KO Validated
human	WB	J Virol; NF-κB-Interacting Long Noncoding RNA Regulates HIV-1 Replication and Latency by Repressing NF-κB Signaling.; Authors - Hong Wang
human	WB	Biochem Biophys Res Commun; PMEPA1, a TGF-β- and hypoxia-inducible gene that participates in hypoxic gene expression networks in solid tumors.; Authors - Masaru Koido ; KD Validated