PML Rekombinanter Antikörper

PML Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242564C10

Kat-Nr. : 85048-5-RR

Synonyme

242564C10, E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML

---

• 

  IF Staining of HeLa using 85048-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PML antibody (85048-5-RR, Clone: 242564C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of A431 using 85048-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug PML Recombinant antibody (85048-5-RR, Clone:242564C10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 85048-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85048-5-RR against Human PML were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85048-5-RR bindet in IF/ICC, FC (Intra), ELISA PML und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PML fusion protein Ag15377
• Vollständiger Name: promyelocytic leukemia
• Berechnetes Molekulargewicht: 882 aa, 98 kDa
• GenBank-Zugangsnummer: BC020994
• Gene symbol: PML
• Gene ID (NCBI): 5371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.