PMM1 Polyklonaler Antikörper

PMM1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 11252-2-AP

Synonyme

phosphomannomutase 1, PMM 1, PMM1, PMMH 22, PMMH22, Sec53

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  WB analysis of mouse brain using 11252-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11252-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11252-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11252-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11252-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen, humanes Hirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11252-2-AP bindet in WB, IHC, ELISA PMM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PMM1 fusion protein Ag1782
• Vollständiger Name: phosphomannomutase 1
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC016818
• Gene symbol: PMM1
• Gene ID (NCBI): 5372
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMM1, also named as PMMH22, belongs to the eukaryotic PMM family. It catalyzes the second step in the conversion of fructose-6P to GDP-mannoseis and is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, PMM1 may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain(PMID:18927083).

Publikationen

Species	Application	Title
mouse	WB	J Proteome Res; Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese.; Authors - Wegrzynowicz Michal M