PMM2 Polyklonaler Antikörper

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PMM2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10666-1-PBS

Synonyme

phosphomannomutase 2, EC:5.4.2.8

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  WB analysis of multi-tissue using 10666-1-AP (same clone as 10666-1-PBS)

  WB results of 10666-1-AP (PMM2 antibody) on several lysates. This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10666-1-AP (same clone as 10666-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10666-1-AP (same clone as 10666-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 10666-1-AP (same clone as 10666-1-PBS)

  IP result of anti-PMM2 (IP:10666-1-AP, 4ug; Detection:10666-1-AP 1:1000) with mouse lung tissue lysate 3600ug. This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10666-1-AP (same clone as 10666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10666-1-AP (same clone as 10666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10666-1-AP (same clone as 10666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10666-1-AP (PMM2 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10666-1-AP (same clone as 10666-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PMM2 antibody (10666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10666-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10666-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PMM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PMM2 fusion protein Ag1031
• Vollständiger Name: phosphomannomutase 2
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC008310
• Gene symbol: PMM2
• Gene ID (NCBI): 5373
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMM2(Phosphomannomutase 2) belongs to the eukaryotic PMM family. It is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM2 catalyzes the second step in the conversion of fructose-6P to GDP-mannose.