PMP2 Polyklonaler Antikörper

PMP2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 12717-1-AP

Synonyme

FABP8, M FABP, MP2, Myelin P2 protein, P2, peripheral myelin protein 2, PMP2

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Galerie der Validierungsdaten

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  WB analysis of pig spinal cord using 12717-1-AP

  pig spinal cord tissue were subjected to SDS PAGE followed by western blot with 12717-1-AP (PMP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12717-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12717-1-AP (PMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12717-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12717-1-AP (PMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12717-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12717-1-AP (PMP2 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Rückenmarkgewebe, humanes Hirngewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

12717-1-AP bindet in WB, IHC, IF, ELISA PMP2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PMP2 fusion protein Ag3411
• Vollständiger Name: peripheral myelin protein 2
• Berechnetes Molekulargewicht: 132 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC034997
• Gene symbol: PMP2
• Gene ID (NCBI): 5375
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMP2 (peripheral myelin protein-2, also known as P2) is one of the major proteins of peripheral myelin and appears to be related to the transport of fatty acids or the metabolism of myelin lipids.P2 constitutes up to 15% of total protein of peripheral nervous system (PNS) myelin. It is also present in small amounts in central nervous system (CNS) myelin, being abundant in spinal cord and brain stem myelin. As a structural protein, P2 is thought to stabilize the myelin membranes.

Publikationen

Species	Application	Title
human	IHC	Nat Neurosci; Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes.; Authors - Kevin W Kelley
mouse	IHC	Nat Neurosci; Disentangling glial diversity in peripheral nerves at single-nuclei resolution.; Authors - Aldrin K Y Yim
pig	WB	Neuron; Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.; Authors - Nadav I Weinstock
mouse	WB	Elife; Proteome profile of peripheral myelin in healthy mice and in a neuropathy model.; Authors - Sophie B Siems
human,mouse	WB,IHC	Elife; Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.; Authors - Vasiliki-Ilya Gargareta
mouse	IHC	Glia; A role of peripheral myelin protein 2 in lipid homeostasis of myelinating schwann cells.; Authors - Jennifer Zenker