PMS2 Polyklonaler Antikörper

PMS2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18164-1-AP

Synonyme

PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-

---

• 

  WB analysis using 18164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18164-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18164-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 18164-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 18164-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Jurkat-Zellen, MCF-7-Zellen, PC-3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, human colon cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18164-1-AP bindet in WB, IHC, ELISA PMS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PMS2 fusion protein Ag12651
• Vollständiger Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 862 aa, 96 kDa
• Beobachtetes Molekulargewicht: 117 kDa, 100 kDa
• GenBank-Zugangsnummer: BC093921
• Gene symbol: PMS2
• Gene ID (NCBI): 5395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS). This antibody is specific to PMS2. The calculated molecular weight of PMS2 is 96 kDa, but the modified PMS2 is about 117 kDa.(PMID: 11358834)

Publikationen

Species	Application	Title
		Aging (Albany NY); Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation; Authors - Baining Zhang