PMS2 Monoklonaler Antikörper

PMS2 Monoklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Kaninchen

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1A3

Kat-Nr. : 68905-5-Ig

Synonyme

1D1A3, EC:3.1.-.-, HNPCC4, PMS1 protein homolog 2, PMS2CL

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  WB analysis using 68905-5-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68905-5-Ig (PMS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit brain using 68905-5-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68905-5-Ig (PMS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 68905-5-Ig

  IP result of anti-PMS2 (IP:68905-5-Ig, 4ug; Detection:68905-5-Ig 1:1000) with HEK-293 cells lysate 1800 ug.

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  IHC staining of human tonsillitis using 68905-5-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68905-5-Ig (PMS2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human colon cancer using 68905-5-Ig

  Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue slide using 68905-5-Ig (PMS2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Kaninchenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68905-5-Ig bindet in WB, IHC, IP, ELISA PMS2 und zeigt Reaktivität mit human, Kaninchen

• Getestete Reaktivität: human, Kaninchen
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PMS2 fusion protein Ag34237
• Vollständiger Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 862 aa, 96 kDa
• Beobachtetes Molekulargewicht: 117 kDa, 100 kDa
• GenBank-Zugangsnummer: BC093921
• Gene symbol: PMS2
• Gene ID (NCBI): 5395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).