PMS2 Monoklonaler Antikörper

PMS2 Monoklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Kaninchen

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1A3

Kat-Nr. : 68905-5-PBS

Synonyme

1D1A3, EC:3.1.-.-, HNPCC4, PMS1 protein homolog 2, PMS2CL

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  WB analysis using 68905-5-Ig (same clone as 68905-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68905-5-Ig (PMS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68905-5-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 68905-5-Ig (same clone as 68905-5-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68905-5-Ig (PMS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68905-5-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 68905-5-Ig (same clone as 68905-5-PBS)

  IP result of anti-PMS2 (IP:68905-5-Ig, 4ug; Detection:68905-5-Ig 1:1000) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 68905-5-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68905-5-Ig (same clone as 68905-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68905-5-Ig (PMS2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68905-5-PBS in a different storage buffer formulation.

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  IHC staining of Human colon cancer using 68905-5-Ig (same clone as 68905-5-PBS)

  Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue slide using 68905-5-Ig (PMS2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68905-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68905-5-PBS bindet in WB, IHC, IP, Indirect ELISA PMS2 und zeigt Reaktivität mit human, Kaninchen

• Getestete Reaktivität: human, Kaninchen
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PMS2 fusion protein Ag34237
• Vollständiger Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 862 aa, 96 kDa
• Beobachtetes Molekulargewicht: 117 kDa, 100 kDa
• GenBank-Zugangsnummer: BC093921
• Gene symbol: PMS2
• Gene ID (NCBI): 5395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).