PMS2 Rekombinanter Antikörper

PMS2 Rekombinant Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242455F8

Kat-Nr. : 84894-5-PBS

Synonyme

242455F8, EC:3.1.-.-, HNPCC4, PMS1 protein homolog 2, PMS2CL

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  WB analysis using 84894-5-RR (same clone as 84894-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84894-5-RR (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84894-5-RR (same clone as 84894-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84894-5-RR (same clone as 84894-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 84894-5-RR (same clone as 84894-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 84894-5-RR (same clone as 84894-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PMS2 Recombinant antibody (84894-5-RR, Clone:242455F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84894-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84894-5-RR against Human PMS2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84894-5-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA PMS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PMS2 fusion protein Ag33508
• Vollständiger Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 862 aa, 96 kDa
• Beobachtetes Molekulargewicht: 117 kDa
• GenBank-Zugangsnummer: BC093921
• Gene symbol: PMS2
• Gene ID (NCBI): 5395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).