PMS2 Rekombinanter Antikörper

PMS2 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242455F8

Kat-Nr. : 84894-5-RR

Synonyme

242455F8, EC:3.1.-.-, HNPCC4, PMS1 protein homolog 2, PMS2CL

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  WB analysis using 84894-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84894-5-RR (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 84894-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84894-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 84894-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 84894-5-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PMS2 Recombinant antibody (84894-5-RR, Clone:242455F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84894-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84894-5-RR against Human PMS2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, Caco-2-Zellen, HEK-293T-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Tonsillengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84894-5-RR bindet in WB, IHC, FC (Intra), ELISA PMS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PMS2 fusion protein Ag33508
• Vollständiger Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 862 aa, 96 kDa
• Beobachtetes Molekulargewicht: 117 kDa
• GenBank-Zugangsnummer: BC093921
• Gene symbol: PMS2
• Gene ID (NCBI): 5395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).