PNMA5 Polyklonaler Antikörper

PNMA5 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21770-1-PBS

Synonyme

KIAA1934, paraneoplastic antigen like 5

---

• 

  WB analysis using 21770-1-AP (same clone as 21770-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21770-1-AP (PNMA5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 21770-1-AP (same clone as 21770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21770-1-AP (PNMA5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 21770-1-AP (same clone as 21770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21770-1-AP (PNMA5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21770-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 21770-1-AP (same clone as 21770-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PNMA5 antibody (21770-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21770-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

21770-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA PNMA5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PNMA5 fusion protein Ag16414
• Vollständiger Name: paraneoplastic antigen like 5
• Berechnetes Molekulargewicht: 448 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC101112
• Gene symbol: PNMA5
• Gene ID (NCBI): 114824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PNMA5 is a member of the paraneoplastic Ma antigen (PNMA) family proteins which share relatively conserved protein sequences and are involved in the regulation of growth, apoptosis, or fate determination in different cell types (PMID: 29378289). PNMA5 appears to be involved in the association areas of the neocortex during primate evolution (PMID: 19366867). PNMA5 has been reported to play roles in the pathological development and metastasis of some cancers, including colorectal cancer, ovarian cancer, and lung cancer (PMID: 35559417; 35117731; 34136487).