PNMT Rekombinanter Antikörper

PNMT Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241111G1

Kat-Nr. : 83979-5-RR

Synonyme

241111G1, EC:2.1.1.28, Noradrenaline N-methyltransferase, PENT, Phenylethanolamine N-methyltransferase

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  WB analysis of K-562 using 83979-5-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83979-5-RR (PNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83979-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83979-5-RR against Human PNMT were performed. The affinity constant is 8.83 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83979-5-RR bindet in WB, ELISA PNMT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PNMT fusion protein Ag4098
• Vollständiger Name: phenylethanolamine N-methyltransferase
• Berechnetes Molekulargewicht: 282 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC037246
• Gene symbol: PNMT
• Gene ID (NCBI): 5409
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PNMT (Phenylethanolamine N-methyltransferase) is the final enzyme in the catecholamine synthesizing cascade that converts noradrenaline to epinephrine by transferring a methyl group from S-adenosyl-L-methionine (SAM) to norepinephrine. PNMT is primarily found in the adrenal medulla, where it is responsible for the production of epinephrine. It is also present in certain areas of the brain, such as the amygdala and retina, where it may be involved in various neurophysiological functions (PMID: 17175506). Variations in the PNMT gene have been associated with Alzheimer's disease, hypertension, and other disorders (PMID: 2816488,.11514309).