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PNO1 Rekombinanter Antikörper

PNO1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250134F6

Kat-Nr. : 85834-4-PBS

Synonyme

KHRBP1, Partner of NOB1, RNA binding protein PNO1, RNA-binding protein PNO1

Filter:
  • Western Blot (WB) analysis of various lysates using PNO1 Recombinant antibody (85834-4-RR)

    WB analysis using 85834-4-RR (same clone as 85834-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85834-4-RR (PNO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85834-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PNO1 Recombinant antibody (85834-4-RR)

    IF Staining of A549 using 85834-4-RR (same clone as 85834-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PNO1 antibody (85834-4-RR, Clone: 250134F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85834-4-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85834-4-PBS bindet in WB, IF/ICC, Indirect ELISA PNO1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PNO1 fusion protein Ag14752
Vollständiger Name partner of NOB1 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 252 aa, 28 kDa
Beobachtetes Molekulargewicht 28-35 kDa
GenBank-ZugangsnummerBC008304
Gene symbol PNO1
Gene ID (NCBI) 56902
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human RNA binding gene partner of NOB1 (PNO1) was firstly isolated from human kidney and encodes 35-kDa protein, named PNO1. PNO1 as a ribosome biogenesis factor that contributes to 40S subunit assembly by binding with its partner NOB1. PNO1 is a highly conserved protein with a K homology (KH) domain at its C-terminal and two putative nuclear localization signals at its N-terminal . PNO1 also had great regulatory function in tumor metabolism. (PMID: 38071381, PMID: 33864661)