PNPLA3 Monoklonaler Antikörper

PNPLA3 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2B11

Kat-Nr. : 67369-1-PBS

Synonyme

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3

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  WB analysis using 67369-1-Ig (same clone as 67369-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67369-1-Ig (PNPLA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67369-1-Ig (PNPLA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67369-1-Ig (PNPLA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67369-1-Ig (same clone as 67369-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPLA3 antibody (67369-1-Ig, Clone: 1D2B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67369-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PNPLA3 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PNPLA3 fusion protein Ag27407
• Vollständiger Name: patatin-like phospholipase domain containing 3
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC065195
• Gene symbol: PNPLA3
• Gene ID (NCBI): 80339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)