PNPLA6 Polyklonaler Antikörper

PNPLA6 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14261-1-PBS

Synonyme

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  WB analysis of HEK-293 using 14261-1-AP (same clone as 14261-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 14261-1-AP (same clone as 14261-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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  IP experiment using 14261-1-AP (same clone as 14261-1-PBS)

  IP result of anti-PNPLA6 (14261-1-AP for IP and Detection) with HEK-293 cells. This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14261-1-AP (same clone as 14261-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14261-1-AP (same clone as 14261-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14261-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA PNPLA6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PNPLA6 fusion protein Ag5546
• Vollständiger Name: patatin-like phospholipase domain containing 6
• Berechnetes Molekulargewicht: 150 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC051768
• Gene symbol: PNPLA6
• Gene ID (NCBI): 10908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PNPLA6(patatin-like phospholipase domain-containing protein 6) is also named as NTE(neuropathy target esterase) and belongs to the NTE family. It plays an important role in motor neuron development and implicated overexpression of BMP signaling(PMID:22996643). Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39)(PMID:18313024). This protein has a glycosylaion site that it can be glycosylated(PMID:9576844). It has 3 isoforms produced by alternative splicing.