PNPO Polyklonaler Antikörper

PNPO Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 15552-1-AP

Synonyme

Pyridoxine-5'-phosphate oxidase, Pyridoxine 5 phosphate oxidase, Pyridoxamine-phosphate oxidase, Pyridoxamine phosphate oxidase, PDXPO

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  WB analysis using 15552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15552-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15552-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15552-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 15552-1-AP

  IP result of anti-PNPO (IP:15552-1-AP, 4ug; Detection:15552-1-AP 1:500) with MCF-7 cells lysate 3200 ug.

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  IHC staining of human liver using 15552-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15552-1-AP (PNPO antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15552-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15552-1-AP (PNPO antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15552-1-AP

  Immunofluorescent analysis of Hela cells, using PNPO antibody 15552-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Hirngewebe, humanes Herzgewebe, humanes Lebergewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below

Produktinformation

15552-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PNPO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PNPO fusion protein Ag7914
• Vollständiger Name: pyridoxamine 5'-phosphate oxidase
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC006525
• Gene symbol: PNPO
• Gene ID (NCBI): 55163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse		Nat Metab; Spatial hepatocyte plasticity of gluconeogenesis during the metabolic transitions between fed, fasted and starvation states; Authors - Junichi Okada
human	IHC	Front Cell Dev Biol; Prognostic Implication and Oncogenic Role of PNPO in Pan-Cancer.; Authors - Lingyun Zhang
	WB	bioRxiv; Patient mutations in DRP1 perturb synaptic maturation of cortical neurons; Authors - T B Baum
mouse	WB,IHC	Adv Sci (Weinh); PNPO-Mediated Oxidation of DVL3 Promotes Multiple Myeloma Malignancy and Osteoclastogenesis by Activating the Wnt/β-Catenin Pathway; Authors - Zhendong Deng
mouse	IHC	Apoptosis; Targeting PNPO to suppress tumor growth via inhibiting autophagic flux and to reverse paclitaxel resistance in ovarian cancer; Authors - Xin Li
mouse	WB	Nat Metab; PNPO-PLP axis senses prolonged hypoxia in macrophages by regulating lysosomal activity; Authors - Hiroki Sekine