PNPT1 Monoklonaler Antikörper

PNPT1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3F2A1

Kat-Nr. : 68309-1-Ig

Synonyme

3 5 RNA exonuclease OLD35, DKFZp762K1914, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1

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Galerie der Validierungsdaten

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  WB analysis using 68309-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68309-1-Ig (PNPT1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68309-1-Ig bindet in WB, ELISA PNPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PNPT1 fusion protein Ag6290
• Vollständiger Name: polyribonucleotide nucleotidyltransferase 1
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC053660
• Gene symbol: PNPT1
• Gene ID (NCBI): 87178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.