PNPT1 Monoklonaler Antikörper

PNPT1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F2A1

Kat-Nr. : 68309-1-PBS

Synonyme

3'-5' RNA exonuclease OLD35, 3F2A1, EC:2.7.7.8, old 35, OLD35

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  WB analysis using 68309-1-Ig (same clone as 68309-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68309-1-Ig (PNPT1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPT1 antibody (68309-1-Ig, Clone: 3F2A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68309-1-Ig (same clone as 68309-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.2 μg PNPT1 Monoclonal antibody (68309-1-Ig, Clone:3F2A1, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone:1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  FC experiment of LNCaP using 68309-1-Ig (same clone as 68309-1-PBS)

  1x10^6 LNCaP cells were intracellularly stained with 0.2 μg PNPT1 Monoclonal antibody (68309-1-Ig, Clone:3F2A1, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone:1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68309-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA PNPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PNPT1 fusion protein Ag6290
• Vollständiger Name: polyribonucleotide nucleotidyltransferase 1
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC053660
• Gene symbol: PNPT1
• Gene ID (NCBI): 87178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.