POFUT2 Polyklonaler Antikörper

POFUT2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 17764-1-AP

Synonyme

C21orf80, FUT13, KIAA0958, O FucT 2, Peptide O fucosyltransferase 2, POFUT2, protein O fucosyltransferase 2

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 17764-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17764-1-AP (POFUT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17764-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17764-1-AP (POFUT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 17764-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17764-1-AP (POFUT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17764-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17764-1-AP (POFUT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 17764-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17764-1-AP (POFUT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17764-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17764-1-AP (POFUT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17764-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17764-1-AP (POFUT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

17764-1-AP bindet in WB, IHC, ELISA POFUT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POFUT2 fusion protein Ag12148
• Vollständiger Name: protein O-fucosyltransferase 2
• Berechnetes Molekulargewicht: 429 aa, 50 kDa
• Beobachtetes Molekulargewicht: 49-50 kDa
• GenBank-Zugangsnummer: BC064623
• Gene symbol: POFUT2
• Gene ID (NCBI): 23275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	EMBO Mol Med; A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder; Authors - Ren e G Feichtinger
human	IHC	Cell Death Dis; LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.; Authors - S Liu
		Schizophr Res; Altered fucosyltransferase expression in the superior temporal gyrus of elderly patients with schizophrenia.; Authors - Toni M Mueller
human	WB	Dev Biol; Genetic and biochemical evidence that gastrulation defects in Pofut2 mutants result from defects in ADAMTS9 secretion.; Authors - Brian A Benz
		Methods Mol Biol; Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays.; Authors - Steven John Berardinelli
human	WB	J Cancer Res Clin Oncol; Emerging glyco-risk prediction model to forecast response to immune checkpoint inhibitors in colorectal cancer; Authors - Peishan Qiu ; KD Validated