POLA2 Polyklonaler Antikörper

POLA2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21778-1-AP

Synonyme

DNA polymerase alpha subunit B

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• 

  WB analysis of HepG2 using 21778-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21778-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21778-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21778-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21778-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21778-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21778-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21778-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 21778-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21778-1-AP (POLA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

21778-1-AP bindet in WB, IHC, IF/ICC, ELISA POLA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLA2 fusion protein Ag0271
• Vollständiger Name: polymerase (DNA directed), alpha 2 (70kD subunit)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC001347
• Gene symbol: POLA2
• Gene ID (NCBI): 23649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; CST does not evict elongating telomerase but prevents initiation by ssDNA binding.; Authors - Arthur J Zaug
human	IHC	Cancer Cell Int; Overexpression of POLA2 in hepatocellular carcinoma is involved in immune infiltration and predicts a poor prognosis; Authors - Long Liu
human	WB	Transl Cancer Res; E2F-mediated POLA2 upregulation is correlated with macrophage infiltration and poor prognosis in hepatocellular carcinoma; Authors - Yao Teng