POLD1 Polyklonaler Antikörper

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POLD1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15646-1-PBS

Synonyme

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  WB analysis using 15646-1-AP (same clone as 15646-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15646-1-AP (POLD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15646-1-AP (same clone as 15646-1-PBS)

  IP result of anti-POLD1 (IP:15646-1-AP, 4ug; Detection:15646-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15646-1-PBS bindet in WB, IHC, IP, Indirect ELISA POLD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLD1 fusion protein Ag8178
• Vollständiger Name: polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• Berechnetes Molekulargewicht: 1107 aa, 124 kDa
• Beobachtetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC008800
• Gene symbol: POLD1
• Gene ID (NCBI): 5424
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (PMID: 30344713 )