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POLD1 Rekombinanter Antikörper

POLD1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250828E5

Kat-Nr. : 86197-2-PBS

Synonyme

3'-5' exodeoxyribonuclease, CDC2, EC:2.7.7.7, EC:3.1.11.-, POLD

Filter:
  • Western Blot (WB) analysis of various lysates using POLD1 Recombinant antibody (86197-2-RR)

    WB analysis using 86197-2-RR (same clone as 86197-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86197-2-RR (POLD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86197-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLD1 Recombinant antibody (86197-2-RR)

    IF Staining of HeLa using 86197-2-RR (same clone as 86197-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD1 antibody (86197-2-RR, Clone: 250828E5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86197-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86197-2-RR

    Affinity and Kinetic Characterization of 86197-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86197-2-RR against Human POLD1 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

86197-2-PBS bindet in WB, IF/ICC, Indirect ELISA POLD1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen POLD1 fusion protein Ag8178
Vollständiger Name polymerase (DNA directed), delta 1, catalytic subunit 125kDa
Berechnetes Molekulargewicht 1107 aa, 124 kDa
Beobachtetes Molekulargewicht 124 kDa
GenBank-ZugangsnummerBC008800
Gene symbol POLD1
Gene ID (NCBI) 5424
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (