POLD2 Polyklonaler Antikörper

POLD2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29765-1-PBS

Synonyme

DNA polymerase delta subunit 2

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  WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 29765-1-AP (same clone as 29765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 29765-1-AP (same clone as 29765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 29765-1-AP (same clone as 29765-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD2 antibody (29765-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29765-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA POLD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLD2 fusion protein Ag31521
• Vollständiger Name: polymerase (DNA directed), delta 2, regulatory subunit 50kDa
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 48-51 kDa
• GenBank-Zugangsnummer: BC000459
• Gene symbol: POLD2
• Gene ID (NCBI): 5425
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.