POLDIP2 Polyklonaler Antikörper

POLDIP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 15080-1-AP

Synonyme

HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2

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  WB analysis using 15080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15080-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15080-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15080-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15080-1-AP

  IP result of anti-POLDIP2 (IP:15080-1-AP, 4ug; Detection:15080-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human stomach cancer using 15080-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15080-1-AP (POLDIP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15080-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15080-1-AP (POLDIP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15080-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLDIP2 antibody (15080-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

15080-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA POLDIP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLDIP2 fusion protein Ag7154
• Vollständiger Name: polymerase (DNA-directed), delta interacting protein 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000655
• Gene symbol: POLDIP2
• Gene ID (NCBI): 26073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).

Publikationen

Species	Application	Title
	WB	EMBO J; Poldip2 promotes mtDNA elimination during Drosophila spermatogenesis to ensure maternal inheritance; Authors - Ziming Wang
mouse	WB	Proc Natl Acad Sci U S A; DNA polymerase δ-interacting protein 2 is a processivity factor for DNA polymerase λ during 8-oxo-7,8-dihydroguanine bypass.; Authors - Maga Giovanni G
human	IF	Cell Cycle; PDIP38 is translocated to the spliceosomes/nuclear speckles in response to UV-induced DNA damage and is required for UV-induced alternative splicing of MDM2.; Authors - Agnes Wong
mouse,human	WB	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key
mouse,rat	WB	Life Sci; Poldip2 Aggravates inflammation in diabetic retinopathy by impairing mitophagy via the AMPK/ULK1/Pink1 pathway; Authors - Siyu Lin