POLDIP2 Rekombinanter Antikörper

POLDIP2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250999B11

Kat-Nr. : 86232-3-RR

Synonyme

HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2

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  WB analysis using 86232-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 86232-3-RR

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86232-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLDIP2 antibody (86232-3-RR, Clone: 250999B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86232-3-RR bindet in WB, IF/ICC, ELISA POLDIP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: POLDIP2 fusion protein Ag7154
• Vollständiger Name: polymerase (DNA-directed), delta interacting protein 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC000655
• Gene symbol: POLDIP2
• Gene ID (NCBI): 26073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).