POLG2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

POLG2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 10997-2-AP

Synonyme

DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB

---

• 

  WB analysis of COLO 320 using 10997-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10997-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 10997-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10997-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10997-2-AP (POLG2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of U-251 using 10997-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using POLG2 antibody (10997-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10997-2-AP bindet in WB, IHC, IF/ICC, ELISA POLG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLG2 fusion protein Ag1424
• Vollständiger Name: polymerase (DNA directed), gamma 2, accessory subunit
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC009194
• Gene symbol: POLG2
• Gene ID (NCBI): 11232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

mtDNA is replicated by DNA polymerase gamma, which is composed of a catalytic (encoded by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.

Publikationen

Species	Application	Title
human	WB	Acta Pharm Sin B; Miriplatin-loaded liposome, as a novel mitophagy inducer, suppresses pancreatic cancer proliferation through blocking POLG and TFAM-mediated mtDNA replication; Authors - Xiaowei Wang
mouse	WB	Sci Rep; PMID: 28397817; Authors - Xinbin Zhao
human	WB	Biochim Biophys Acta Mol Cell Res; TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication; Authors - Teppei Inatomi
human	WB	Mitochondrion; The accessory subunit of human DNA polymerase γ is required for mitochondrial DNA maintenance and is able to stabilize the catalytic subunit.; Authors - Yura Do ; KD Validated
human	IF	Nat Cell Biol; Mitochondrial DNA replication stress triggers a pro-inflammatory endosomal pathway of nucleoid disposal; Authors - Laura E Newman
human	WB,IHC	PLoS One; Interference with mitochondrial metabolism could serve as a potential therapeutic strategy for advanced prostate cancer; Authors - Chuang Wu