POLG2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

POLG2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10997-2-PBS

Synonyme

DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB

---

• 

  WB analysis of COLO 320 using 10997-2-AP (same clone as 10997-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10997-2-AP (same clone as 10997-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 10997-2-AP (same clone as 10997-2-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10997-2-AP (same clone as 10997-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10997-2-AP (POLG2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 10997-2-AP (same clone as 10997-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using POLG2 antibody (10997-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

10997-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA POLG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLG2 fusion protein Ag1424
• Vollständiger Name: polymerase (DNA directed), gamma 2, accessory subunit
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC009194
• Gene symbol: POLG2
• Gene ID (NCBI): 11232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

mtDNA is replicated by DNA polymerase gamma, which is composed of a catalytic (encoded by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.