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POLG2 Rekombinanter Antikörper

POLG2 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250198G10

Kat-Nr. : 85852-4-PBS

Synonyme

DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB

Filter:
  • Western Blot (WB) analysis of various lysates using POLG2 Recombinant antibody (85852-4-RR)

    WB analysis using 85852-4-RR (same clone as 85852-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85852-4-RR (POLG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85852-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using POLG2 Recombinant antibody (85852-4-RR)

    WB analysis using 85852-4-RR (same clone as 85852-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85852-4-RR (POLG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85852-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85852-4-RR

    Affinity and Kinetic Characterization of 85852-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85852-4-RR against Human POLG2 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85852-4-PBS bindet in WB, Indirect ELISA POLG2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen POLG2 fusion protein Ag1424
Vollständiger Name polymerase (DNA directed), gamma 2, accessory subunit
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht52-55 kDa
GenBank-ZugangsnummerBC009194
Gene symbol POLG2
Gene ID (NCBI) 11232
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in cell lines with a single band of 52-55kd.