POLH Polyklonaler Antikörper

POLH Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 28133-1-AP

Synonyme

DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV

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• 

  WB analysis of BxPC-3 using 28133-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 28133-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 28133-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 28133-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, COLO 320-Zellen, HEK-293T-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

28133-1-AP bindet in WB, ELISA POLH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLH fusion protein Ag27973
• Vollständiger Name: polymerase (DNA directed), eta
• Berechnetes Molekulargewicht: 713aa,78 kDa; 414aa,46 kDa
• Beobachtetes Molekulargewicht: 78-85 kDa
• GenBank-Zugangsnummer: BC015742
• Gene symbol: POLH
• Gene ID (NCBI): 5429
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	J Tradit Chin Med; Integrating network pharmacology and experimental validation to uncover the synergistic effects of Huangqi ()-Ezhu () with 5-fluorouracil in colorectal cancer models; Authors - Tan Xiying

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katherine (Verified Customer) (10-31-2023)

The quality was good. Band was around 70kD

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 293T

POLH Antibody Western Blot validation (1:500 dilution) in 293T (Cat no:28133-1-AP)