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POLH Polyklonaler Antikörper

POLH Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28133-1-AP

Synonyme

DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of BxPC-3 cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of BxPC-3 using 28133-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of HeLa using 28133-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of COLO 320 using 28133-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of HEK-293T using 28133-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBBxPC-3-Zellen, COLO 320-Zellen, HEK-293T-Zellen, HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

28133-1-AP bindet in WB,ELISA POLH und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen POLH fusion protein Ag27973
Vollständiger Name polymerase (DNA directed), eta
Berechnetes Molekulargewicht713aa,78 kDa; 414aa,46 kDa
Beobachtetes Molekulargewicht 78-85 kDa
GenBank-ZugangsnummerBC015742
Gene symbol POLH
Gene ID (NCBI) 5429
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for POLH antibody 28133-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Katherine (Verified Customer) (10-31-2023)

The quality was good. Band was around 70kD

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: 293T
POLH Antibody Western Blot validation (1:500 dilution) in 293T (Cat no:28133-1-AP)